What speed should a centrifuge be set for PCV centrifugation?

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Multiple Choice

What speed should a centrifuge be set for PCV centrifugation?

Explanation:
For PCV, you need enough centrifugal force to push red blood cells to the bottom of the tube quickly, leaving a distinct plasma layer on top. This is achieved with a high-speed, short spin. About 10,000 rpm for five minutes is a widely used setting for microhematocrit PCV, giving a clean separation without overloading or damaging the cells. Slower speeds can fail to separate the layers adequately within five minutes, while much higher speeds risk hemolysis or tube damage and can distort the interface. So the best choice is 10,000 rpm for five minutes.

For PCV, you need enough centrifugal force to push red blood cells to the bottom of the tube quickly, leaving a distinct plasma layer on top. This is achieved with a high-speed, short spin. About 10,000 rpm for five minutes is a widely used setting for microhematocrit PCV, giving a clean separation without overloading or damaging the cells. Slower speeds can fail to separate the layers adequately within five minutes, while much higher speeds risk hemolysis or tube damage and can distort the interface. So the best choice is 10,000 rpm for five minutes.

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